Phage RBP Database

Genbank accession

WAQ79264.1 [GenBank]

Protein name

tail spike protein

RBP type

TF

Evidence Phold

Probability 1,00

TSP

Evidence DepoScope

Probability 1,00

TSP

Evidence RBPdetect

Probability 0,91

TSP

Evidence RBPdetect2

Probability 0,99

Seq2Symm

C3 confidence 1,000

C3	1,000
C1	0,072
D3	0,007

Predicted homo-oligomer symmetry (Seq2Symm, per-class probabilities; C1 = monomer, C2 = dimer, C3 = trimer).

Protein sequence

MNEMFSQGGKGSTGILTNKQAVARHFGVKQSEVVYFSTGAVLSGYKVIYDKASQRAYSLPADIGSGVTAVSLSPAGVLVHSAGNVDLGALAVERKEYINSPGSFTTGATLTVKNQRLIHNGEYYIWNGAFPKVVPASSTPENSGGVANDAWSKLSPLVKSEEVPYVRMPNAINTNVAANMDLMGRRVIYATDFGVTVDSADNADALWELGQYLSTQVTEPVKVIFPAGTSLVGSQYLTGSTGQGGSYKPSYEQRAWTDASAKGWFSIHMTDANIELEMSNWTLKINDGMRLGAFDPVTGSIAPDVVAETPDYSYMAYQGFLIKLYKAPNVVINGGTSDGNLASAVWGGKFGNTGYQIPCYNMWINQSAGARVYRHKYLNSPVDGLYHQSTGSFSFLDIVPRTVIEDCYWDSCGRNCYSLTGGANIDIINPVITRSGNKAGGIGTHYTGPEAGIDIEAEGGNPYNIRVINPKIVNTGKCAFQTVSVPGTVNDILVVGGVLHSMHSEGAVSNAGNARNIKFVGTTIIGSIIDTGWPAAMGFECYSFIDCELQNRYANDYADEYRLNFKVKEFKGNTITFGIPPTINTNHATINIEDQDATPFGLYAERFKENRLIVYGDASKVTFANGLGGIRNFKNAELYVSADGLTGGTLKITVDTSSAAMNGLSTNTANFNFDVAMDKDVGKNVWYARKVNRVAGVMTAVTDSLQDIGSKDGRFGTMYATKGIILRDVGDSTYKRLRSNNGVLEVVADNT

Physico‐chemical properties

protein length:	751 AA
molecular weight:	80709,62170 Da
isoelectric point:	5,89030
aromaticity:	0,09854
hydropathy:	-0,17004

Domain architecture

WAQ79264.1

1 751 aa

ATT 1–154 · STR 255–525 ·

ATT Attachment Domain STR Structural Domain RBD Receptor-Binding Domain CBM Carbohydrate-Binding Module LEC Lectin-like Domain ENZ Enzymatic Domain CHP Intramolecular Chaperone LNK Linker/Spacer Domain TAS Tail-Associated Structural TTP Tail Tubular Protein UNK Uncharacterized Domain Unmapped

InterPro domains

G3DSA:3.30.2020.50
ATT

G3DSA:3.30.2020.50 G3DSA:2.10.10.80 IPR011050

G3DSA:3.30.2020.50
ATT

G3DSA:3.30.2020.50

IPR040775
RBD

IPR040775

WAQ79264.1

1751 aa

ATT Attachment Domain STR Structural Domain RBD Receptor-Binding Domain CBM Carbohydrate-Binding Module LEC Lectin-like Domain ENZ Enzymatic Domain CHP Intramolecular Chaperone LNK Linker/Spacer Domain TAS Tail-Associated Structural TTP Tail Tubular Protein UNK Uncharacterized Domain Unmapped

Novel HMM domains (de novo, PhaRBP-HMM)

Experimental de novo families on regions without InterPro annotation. Separate from the InterPro architecture above.

DC_1280
ATT

DC_1280 DC_1849

WAQ79264.1

1751 aa

InterPro domain annotations

Domain ID	Category	Cluster	Start	End	Layer	Name	Pref Zone	Confidence	Evidence	Support
G3DSA:3.30.2020.50	ATT	G3DSA:3.30.2020.50	1	96	Merged direct domain	G3DSA:3.30.2020.50	N-terminal	Low	Positional only	289 proteins / 289 hits
G3DSA:3.30.2020.50	ATT	G3DSA:3.30.2020.50	1	100	Merged direct domain	G3DSA:3.30.2020.50	N-terminal	Low	Positional only	289 proteins / 289 hits
IPR040775	RBD	DC_0821	91	154	InterPro cluster	Tail spike TSP1/Gp66, N-terminal domain	Central	High	Text match	1281 proteins / 1285 hits
G3DSA:2.10.10.80	ATT	G3DSA:2.10.10.80	97	153	Merged direct domain	G3DSA:2.10.10.80	N-terminal	Low	Positional only	383 proteins / 383 hits
IPR011050	STR	IPR011050	255	525	Merged direct domain	Pectin lyase fold/virulence factor	Central	Low	Positional only	572 proteins / 572 hits

Proteins with similar domain architecture

Segmented into three structural domains: N-terminal, central, and C-terminal.

Domain layout

WAQ79264.1

1 751 aa

Domain	Start	End	Length (AA)	Confidence
N-terminal	1	198	198	0,9925
Central domain	199	680	483	0,9830
C-terminal	681	751	70	0,9222

N-terminal Central domain C-terminal

View these domains on the 3D structure via the Color by → Tail spike option in the Tertiary structure section below.

Phage

Escherichia phage E20 [NCBI] · taxon 3003230

Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes

Host

Escherichia sp. [NCBI] · taxon 1884818

cellular organisms Bacteria Pseudomonadati Pseudomonadota Gammaproteobacteria Enterobacterales

Genbank protein accession

WAQ79264.1 [NCBI]

Genbank nucleotide accession

OP745616.1 [NCBI]

CDS location

range 61169 -> 63424
strand -

CDS

ATGAACGAAATGTTTTCACAAGGTGGTAAGGGTTCAACTGGAATCTTAACCAACAAACAAGCAGTAGCCCGTCACTTTGGGGTTAAGCAATCTGAGGTTGTTTACTTCTCTACTGGTGCTGTACTAAGCGGTTATAAAGTCATCTATGACAAAGCGTCACAGCGTGCTTACTCCTTACCTGCTGATATTGGTTCAGGTGTTACTGCTGTAAGTCTTAGTCCTGCTGGGGTATTAGTTCACTCTGCTGGTAATGTTGACTTAGGTGCTCTGGCTGTAGAACGTAAGGAGTATATTAACTCTCCCGGTTCATTTACTACTGGGGCTACCCTTACCGTTAAAAACCAAAGACTGATTCATAATGGTGAGTATTATATTTGGAATGGTGCATTCCCTAAAGTTGTCCCGGCATCCTCTACACCAGAAAACTCAGGTGGTGTAGCTAATGATGCCTGGAGTAAGTTAAGCCCATTAGTTAAGTCTGAAGAAGTTCCCTATGTTCGTATGCCTAATGCAATTAATACCAACGTAGCTGCTAACATGGATTTAATGGGTAGACGTGTAATCTATGCTACGGATTTTGGTGTAACTGTTGACTCTGCTGATAACGCAGACGCTCTTTGGGAATTGGGTCAATACTTAAGCACACAGGTAACTGAACCGGTTAAGGTGATATTCCCTGCTGGCACTAGTTTAGTAGGTTCCCAGTATCTCACTGGTTCTACTGGTCAGGGTGGTTCTTATAAACCTTCCTATGAGCAGCGTGCCTGGACAGATGCTTCTGCTAAAGGCTGGTTCTCCATTCATATGACGGATGCTAATATCGAACTGGAGATGAGTAACTGGACACTTAAGATTAATGACGGTATGCGTTTAGGGGCTTTTGATCCTGTTACTGGTTCTATTGCACCGGATGTTGTAGCTGAAACCCCTGATTATAGCTATATGGCTTACCAAGGTTTTCTTATTAAACTGTACAAAGCACCTAACGTCGTAATTAATGGTGGGACCAGTGATGGTAACTTAGCCTCTGCTGTATGGGGGGGTAAGTTTGGTAACACTGGTTATCAGATTCCTTGTTATAACATGTGGATTAACCAGTCAGCAGGTGCTCGTGTTTACCGACATAAATACCTTAATTCGCCTGTAGATGGCTTATATCATCAATCTACCGGTAGCTTCAGTTTCTTGGATATTGTTCCACGCACTGTTATTGAAGACTGTTACTGGGATTCCTGTGGGCGCAACTGCTACTCCCTGACTGGTGGAGCTAACATTGATATCATTAATCCAGTAATTACCCGCTCAGGCAATAAAGCTGGTGGGATTGGTACTCATTATACCGGACCGGAAGCAGGTATTGATATTGAAGCTGAAGGGGGAAACCCTTACAACATTCGTGTTATTAACCCTAAAATTGTTAACACCGGTAAGTGTGCATTCCAGACTGTATCCGTACCGGGGACTGTTAACGATATTCTGGTTGTGGGTGGCGTGCTACATAGTATGCACTCTGAGGGTGCTGTATCCAATGCTGGTAATGCCCGTAATATTAAGTTTGTAGGCACAACCATCATAGGCAGTATTATTGATACTGGCTGGCCTGCTGCTATGGGTTTTGAGTGTTATAGCTTTATTGATTGTGAACTTCAGAACAGATATGCAAATGATTATGCTGATGAGTATCGTTTGAATTTCAAAGTTAAAGAGTTTAAAGGGAATACTATTACATTTGGTATCCCACCCACCATTAACACTAACCATGCAACAATTAATATTGAAGATCAGGACGCTACTCCTTTCGGTCTTTACGCAGAACGATTCAAAGAGAATCGGTTAATTGTTTATGGTGATGCATCTAAAGTTACCTTTGCCAATGGTCTAGGTGGCATTCGTAACTTTAAGAATGCAGAGTTGTACGTTTCTGCTGATGGACTTACTGGAGGTACTCTCAAGATTACTGTTGATACTTCTAGTGCAGCAATGAATGGTTTATCTACCAATACTGCAAACTTTAACTTCGATGTTGCTATGGATAAAGATGTTGGCAAAAATGTCTGGTATGCCCGTAAAGTTAATCGTGTTGCAGGGGTTATGACAGCAGTAACAGATTCCTTGCAGGATATTGGTTCTAAGGATGGACGTTTCGGTACTATGTATGCTACCAAAGGGATTATACTCCGAGATGTGGGGGATAGTACTTACAAACGCCTTCGTTCTAATAACGGAGTACTTGAGGTAGTTGCTGATAACACCTAA

Protein

Domains

Tail Spike Domain Segmentation

Taxonomy

Coding sequence (CDS)

Genome Context

Tertiary structure

Predicted Aligned Error (PAE)