Protein

Experimentally validated RBP function shown
View in Explore
Genbank accession
XOE89804.1 [GenBank]
Protein name
DNA stabilization protein
RBP type
TSP
Evidence DepoScope
Probability 1,00
Seq2Symm
C1 confidence 0,974
C1 0,974
C3 0,006
C4 0,002
Predicted homo-oligomer symmetry (Seq2Symm, per-class probabilities; C1 = monomer, C2 = dimer, C3 = trimer).
Protein sequence
MPIQQLPMMKGMGKDFKNADYIDYLPVNMLATPKEILNSSGYLRSFPGITKRYDMNGVSRGVEYNTAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRTSQAVGVNGQLVEYRYDGTVKTVSNWPADSGFTQYELGSVRDITRLRGRYAWSKDGTDSWFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTAGAALYVAQPSLMVQKGIAGTYCKTPFADSYAFISHPATGAPSVYIIGSGQASPIATASIEKIIRSYTAEEMATGVMETLRFDSHELLIIHLPRHVLVYDASSSQNGPQWCVLKTGLYDDVYRGVDFMYEGNQITCGDKSEAVVGQLQFDISSQYDKQQEHLLFTPLFKADNARCFDLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRRLIGFKLRVITKSPVTLSGCQIRLE
Physico‐chemical
properties
protein length:472 AA
molecular weight: 52456,59830 Da
isoelectric point:5,90440
aromaticity:0,10805
hydropathy:-0,28856

Domains

View on InterPro
Domain architecture
XOE89804.1
1 472 aa
STR 4–472
ATT Attachment Domain STR Structural Domain RBD Receptor-Binding Domain CBM Carbohydrate-Binding Module LEC Lectin-like Domain ENZ Enzymatic Domain CHP Intramolecular Chaperone LNK Linker/Spacer Domain TAS Tail-Associated Structural TTP Tail Tubular Protein UNK Uncharacterized Domain Unmapped
Proteins with similar domain architecture

Tail Spike Domain Segmentation

Segmented into three structural domains: N-terminal, central, and C-terminal.

Domain layout
XOE89804.1
1 472 aa
Domain Start End Length (AA) Confidence
N-terminal 1 338 338 0,7769
Central domain 339 461 124 0,1008
C-terminal 462 472 10 0,9835
N-terminal Central domain C-terminal

View these domains on the 3D structure via the Color by → Tail spike option in the Tertiary structure section below.

Taxonomy

Phage
Salmonella phage As3 [NCBI] · taxon 3388311
Salmonella phage As3
Host
Salmonella typhimurium [NCBI] · taxon 90371
Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica

Coding sequence (CDS)

Genbank protein accession
XOE89804.1 [NCBI]
Genbank nucleotide accession
PQ793800 [NCBI]
CDS location
range 3962 -> 5380
strand -
CDS
ATGCCGATTCAACAACTCCCCATGATGAAGGGAATGGGTAAAGACTTCAAGAACGCCGATTATATCGACTATCTGCCAGTGAATATGCTGGCAACACCCAAAGAAATCCTTAACAGCAGCGGCTATCTCCGCTCATTCCCTGGCATTACCAAACGTTATGATATGAACGGCGTATCGCGTGGAGTTGAGTACAACACCGCTCAGAATGCTGTTTATCGTGTTTGTGGTGGCAAGCTCTACAAAGGAGAAAGCGAAGTTGGTGATGTTGCCGGAAGTGGTCGCGTATCAATGGCACATGGTCGGACATCACAGGCGGTAGGCGTTAATGGCCAACTGGTCGAGTATCGCTATGATGGCACGGTTAAAACCGTCTCAAACTGGCCTGCAGACAGCGGATTCACGCAGTATGAGTTAGGTTCAGTGCGTGACATTACGCGCTTACGTGGGCGTTATGCGTGGTCAAAAGACGGCACTGATTCATGGTTTATCACTGACCTCGAAGATGAATCGCATCCTGACCGCTACAGCGCACAATATCGCGCAGAGTCGCAGCCTGACGGCATCATCGGCATCGGAACATGGAGAGACTTCATCGTCTGCTTTGGTTCGTCAACGATAGAGTATTTCTCCCTGACAGGCGCAACCACCGCTGGCGCTGCGTTGTATGTCGCACAGCCATCGTTGATGGTACAGAAGGGCATTGCCGGAACATACTGTAAAACGCCGTTCGCTGATTCATACGCCTTTATCAGTCATCCGGCTACTGGCGCACCTTCCGTCTACATCATCGGTTCAGGGCAGGCATCGCCAATTGCGACCGCCAGTATTGAGAAAATTATCCGCTCATATACCGCTGAAGAAATGGCGACGGGTGTGATGGAGACTTTGCGCTTCGATTCTCATGAGCTTCTGATTATTCATCTCCCTCGCCATGTTCTGGTTTACGACGCATCGTCCAGCCAGAACGGACCTCAGTGGTGTGTGCTGAAAACCGGGCTTTACGATGATGTATATCGCGGCGTCGACTTCATGTACGAAGGAAACCAGATAACGTGCGGCGACAAATCAGAAGCGGTGGTCGGACAATTGCAATTCGACATCAGCAGCCAGTACGACAAACAACAAGAACACCTACTGTTTACGCCCCTTTTCAAAGCAGATAACGCCAGATGCTTCGACCTTGAGGTTGAATCATCCACTGGTGTTGCTCAATACGCTGACCGCCTGTTCCTGTCTGCAACAACTGACGGCATCAATTACGGTCGTGAACAGATGATTGAGCAGAACGAGCCGTTTGTGTACGACAAGCGCGTTTTATGGAAACGTGTTGGGCGCATTCGTCGATTAATCGGATTCAAACTGCGAGTAATCACCAAATCACCAGTAACACTATCCGGGTGTCAAATTCGTCTGGAGTAA

Genome Context

Tertiary structure

XOE89804.1
1 / 6
Structure ID
Source
Method
Resolution
Oligomeric state

Experimental validation

Experimentally validated

A wet-lab result is recorded for this protein, or for its sequence: a solved structure, an experimental GO annotation, a UniProt ECO:0000269 assertion, or a GenBank /experiment= qualifier.

Tier Source Assay Claim Reference
Experimentally validated PDB
8EAP
 structure:EM — ECO:0000006
Experimentally validated PDB
8EB7
 structure:EM — ECO:0000006
Experimentally validated PDB
8TVR
 structure:EM — ECO:0000006
Experimentally validated PDB
8U10
 structure:EM — ECO:0000006
Experimentally validated PDB
8U11
 structure:EM — ECO:0000006
Validated by sequence identity GenBank
DAA00990.1
 experiment:unspecified head completion protein – experimental evidence, no additional details recorded —
Validated by sequence identity GenBank
NP_059633.1
 experiment:unspecified head closure Hc3 – experimental evidence, no additional details recorded —
Validated by sequence identity UniProt
P26749
 ECO:0000269:FUNCTION:37952769 Acts as a tail hub by assembling onto the head-to-tail adapter protein gp4, generating a channel that is sealed by the tail needle protein gp26 (PubMed:37952769). Together with gp4 and gp26, gp10 is required for stabilization of the condensed DNA within the capsid; perhaps by plugging the hole through which the DNA enters PMID 37952769
Validated by sequence identity UniProt
P26749
 ECO:0000269:SUBUNIT:37952769 RBP Monomer (in solution) (PubMed:37952769). Hexamer; assembles as a hexamer onto the dodecamer formed by the head-to-tail adapter gp4 (PubMed:37952769). Interacts with the head-to-tail adapter protein gp4; each gp10 monomer contacts 2 head-to-tail adapter protein gp4 (PubMed:37952769). Interacts with the tail needle protein gp26 (PubMed:37952769). Interacts with the tail spike protein gp9; binds six tail spike trimers (PubMed:37952769) PMID 37952769
Validated by sequence identity UniProt
P26749
 protein_existence:evidence_at_protein_level 1: Evidence at protein level —

Rows marked “Validated by sequence identity” come from a different entry carrying the same sequence — the experiment was not performed on this protein.

Literature

Title Authors Date PMID Source
Surface-mediated Bacteriophage Defense Incurs Fitness Tradeoffs for Interbacterial Antagonism Tsai,C.E., Wang,F.Q., Yang,C.W., Yang,L.L., Nguyen,T.V., Chen,Y.C., Chen,P.Y., Hwang,I.S. and Ting,S.Y. — 40065098 GenBank