Phage RBP Database

Genbank accession

WCS68322.1 [GenBank]

Protein name

tail spike protein

RBP type

TF

Evidence Phold

Probability 1,00

TSP

Evidence DepoScope

Probability 1,00

TSP

Evidence RBPdetect

Probability 0,91

TSP

Evidence RBPdetect2

Probability 1,00

Seq2Symm

C3 confidence 0,997

C3	0,997
C1	0,719
D3	0,006

Predicted homo-oligomer symmetry (Seq2Symm, per-class probabilities; C1 = monomer, C2 = dimer, C3 = trimer).

Protein sequence

MNSYVINPTQWGIVEGTDPVNATVNKDGFIKAIEYAKSEGYYKVTLPKGTYMINGVSNTTTSPEIGAGIRVPGNIEIEITPDTTLKVAPNGSYGYTLFYVAPSEKNVSFSGGGYLVGERYDHDYSFQGKDGQKKSHEWGYGIYFHGAHDGYVEKLKIMDFTGDGIFLGAKGLLNYTGTEYHYCYNIHIEKCFISNNRRNNISVTAAEVVRIKNCIITKAGADDGCAPRFGIDIEGYGEGSIDYEEIRDVLIEGCTFIGNINASVSNFNGYGVRIVNNQADHVINYGSGTDTLISNNTMVRTDGKNTAIQGDGVSSSQKCNNSLIIGNTIKGFNKGIDARGKGVNIIGNIIDEMHESAVGIVAYYADNVTISGNKVMNENGIAFSIDTSSNISLIGNEAYNSSTRGLDIFRSTSIRIKDFHAIGCYSGIRIRSSEVNFLNILVDFNDIENQSYGIDFDAESQVLFDNITVKKSKNLSILGVATKYRLFMKDIKVLESKYIVPIQITGGMGHRFERIYITTNISGGRGLNLVNTEKVLINDLQAYAATEGASMSAPLVSTSSKSTTLANSLYEGKPWMLEDDKQINNIPVAV

Physico‐chemical properties

protein length:	590 AA
molecular weight:	64388,52990 Da
isoelectric point:	5,51278
aromaticity:	0,09492
hydropathy:	-0,21932

Domain architecture

WCS68322.1

1 590 aa

STR 16–484 ·

ATT Attachment Domain STR Structural Domain RBD Receptor-Binding Domain CBM Carbohydrate-Binding Module LEC Lectin-like Domain ENZ Enzymatic Domain CHP Intramolecular Chaperone LNK Linker/Spacer Domain TAS Tail-Associated Structural TTP Tail Tubular Protein UNK Uncharacterized Domain Unmapped

InterPro domains

IPR012334
STR

IPR012334 IPR011050

IPR011050
STR

IPR011050 IPR012334

IPR039448
ENZ

IPR039448 IPR006626 IPR006626 IPR006626 IPR006626 IPR006626 IPR006626 IPR006626

IPR006626
Unmapped

IPR006626 IPR006626 IPR006626 IPR039448

WCS68322.1

1590 aa

ATT Attachment Domain STR Structural Domain RBD Receptor-Binding Domain CBM Carbohydrate-Binding Module LEC Lectin-like Domain ENZ Enzymatic Domain CHP Intramolecular Chaperone LNK Linker/Spacer Domain TAS Tail-Associated Structural TTP Tail Tubular Protein UNK Uncharacterized Domain Unmapped

Novel HMM domains (de novo, PhaRBP-HMM)

Experimental de novo families on regions without InterPro annotation. Separate from the InterPro architecture above.

DC_1312
RBD

DC_1312

DC_0453
RBD

DC_0453

WCS68322.1

1590 aa

InterPro domain annotations

Domain ID	Category	Cluster	Start	End	Layer	Name	Pref Zone	Confidence	Evidence	Support
IPR012334	STR	IPR012334	16	268	Merged direct domain	Pectin lyase fold	Central	Low	Positional only	867 proteins / 867 hits
IPR011050	STR	IPR011050	29	304	Merged direct domain	Pectin lyase fold/virulence factor	Central	Low	Positional only	572 proteins / 572 hits
IPR039448	ENZ	DC_0799	132	228	InterPro cluster	Right handed beta helix domain	C-terminal	High	Text match	178 proteins / 183 hits
IPR006626	Unmapped	-	147	169	InterPro	Parallel beta-helix repeat	-	-	-	-
IPR006626	Unmapped	-	183	205	InterPro	Parallel beta-helix repeat	-	-	-	-
IPR006626	Unmapped	-	206	235	InterPro	Parallel beta-helix repeat	-	-	-	-
IPR006626	Unmapped	-	246	276	InterPro	Parallel beta-helix repeat	-	-	-	-
IPR011050	STR	IPR011050	278	472	Merged direct domain	Pectin lyase fold/virulence factor	Central	Low	Positional only	572 proteins / 572 hits
IPR006626	Unmapped	-	288	317	InterPro	Parallel beta-helix repeat	-	-	-	-
IPR012334	STR	IPR012334	310	484	Merged direct domain	Pectin lyase fold	Central	Low	Positional only	867 proteins / 867 hits
IPR006626	Unmapped	-	319	340	InterPro	Parallel beta-helix repeat	-	-	-	-
IPR039448	ENZ	DC_0799	320	466	InterPro cluster	Right handed beta helix domain	C-terminal	High	Text match	178 proteins / 183 hits
IPR006626	Unmapped	-	365	387	InterPro	Parallel beta-helix repeat	-	-	-	-
IPR006626	Unmapped	-	388	410	InterPro	Parallel beta-helix repeat	-	-	-	-
IPR006626	Unmapped	-	411	432	InterPro	Parallel beta-helix repeat	-	-	-	-
IPR006626	Unmapped	-	437	458	InterPro	Parallel beta-helix repeat	-	-	-	-

Proteins with similar domain architecture

Segmented into three structural domains: N-terminal, central, and C-terminal.

Domain layout

WCS68322.1

1 590 aa

Domain	Start	End	Length (AA)	Confidence
N-terminal	1	19	19	0,9168
Central domain	20	579	561	0,9949
C-terminal	580	590	10	0,3302

Note: Constraints were applied during segmentation.
C-terminal too short, adjusted boundary

N-terminal Central domain C-terminal

View these domains on the 3D structure via the Color by → Tail spike option in the Tertiary structure section below.

Phage

Bacillus phage vB_BsuM-Goe21 [NCBI] · taxon 3026978

Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes

Host

Bacillus subtilis [NCBI] · taxon 1423

cellular organisms Bacteria Bacillati Bacillota Bacilli Bacillales

Genbank protein accession

WCS68322.1 [NCBI]

Genbank nucleotide accession

OM728297.1 [NCBI]

CDS location

range 162734 -> 164506
strand -

CDS

TTGAATAGTTATGTAATCAATCCAACACAATGGGGAATTGTTGAAGGTACAGATCCTGTTAATGCTACTGTTAACAAAGATGGTTTTATTAAAGCTATTGAGTATGCAAAATCAGAAGGTTACTACAAAGTAACATTACCCAAAGGAACCTATATGATTAATGGAGTAAGTAATACAACAACAAGTCCTGAAATTGGTGCTGGAATTCGTGTTCCGGGAAATATAGAAATTGAAATCACCCCTGATACTACATTAAAAGTAGCACCTAATGGTTCTTATGGCTATACTTTATTCTATGTAGCCCCTAGTGAAAAAAATGTTTCATTTTCAGGAGGAGGTTACTTAGTCGGAGAACGTTATGATCATGATTATAGTTTTCAAGGAAAAGATGGGCAAAAGAAAAGTCATGAATGGGGATATGGTATTTACTTTCATGGTGCTCATGATGGCTATGTTGAAAAACTAAAAATCATGGATTTTACAGGTGATGGAATTTTTCTTGGTGCTAAAGGATTACTTAACTATACAGGAACAGAATACCATTATTGTTACAATATTCATATTGAGAAATGTTTTATTTCCAATAATAGACGTAATAATATTTCAGTTACAGCGGCAGAGGTAGTTAGAATTAAGAATTGTATAATTACTAAAGCTGGTGCTGATGATGGTTGTGCCCCTCGTTTTGGTATTGATATTGAAGGTTATGGAGAAGGTTCTATTGACTATGAGGAAATTAGAGATGTACTTATTGAAGGATGTACATTTATAGGGAATATAAATGCTTCAGTAAGTAACTTCAATGGTTATGGTGTACGTATTGTAAACAATCAGGCTGACCATGTAATTAATTATGGAAGTGGTACTGATACACTTATTTCTAATAATACAATGGTTCGTACTGATGGTAAAAATACTGCCATTCAAGGAGATGGTGTATCATCTTCTCAAAAATGTAATAATTCCTTGATTATTGGAAATACAATCAAAGGATTTAATAAAGGTATTGATGCACGAGGAAAAGGTGTTAATATTATTGGTAATATAATTGATGAGATGCATGAATCTGCAGTTGGCATAGTTGCCTATTATGCTGACAATGTAACCATTTCAGGTAATAAAGTAATGAATGAAAATGGAATTGCTTTCTCAATTGATACCTCTTCAAATATTTCTTTAATAGGAAATGAGGCTTATAACTCATCAACAAGAGGTTTAGATATTTTTAGATCAACAAGTATTCGAATTAAAGATTTCCATGCAATAGGATGCTATTCAGGAATTCGAATTAGATCATCAGAAGTGAACTTTTTAAATATTCTTGTGGATTTTAATGACATTGAGAATCAATCTTATGGTATAGATTTCGATGCAGAAAGCCAAGTTCTATTTGATAATATTACTGTCAAAAAATCTAAGAATTTAAGTATACTTGGGGTGGCAACAAAATATCGTTTATTTATGAAGGATATTAAGGTATTAGAATCTAAATATATTGTACCAATTCAAATAACAGGTGGTATGGGTCATCGATTCGAACGTATTTATATTACAACCAATATTTCAGGTGGACGTGGATTAAATTTAGTAAATACTGAAAAAGTATTAATAAATGATCTACAAGCATATGCGGCAACAGAAGGTGCATCTATGTCAGCACCATTAGTCTCAACGTCATCTAAAAGTACTACCTTGGCTAATAGTCTATACGAAGGAAAACCTTGGATGCTTGAAGATGATAAACAGATCAACAATATACCTGTAGCTGTATAA

Protein

Domains

Tail Spike Domain Segmentation

Taxonomy

Coding sequence (CDS)

Genome Context

Tertiary structure

Predicted Aligned Error (PAE)